Summary of Study ST001251
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000838. The data can be accessed directly via it's Project DOI: 10.21228/M8C671 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001251 |
| Study Title | The effects of a training program encompassing cold exposure, breathing exercises, and meditation on plasma metabomics during experimental human endotoxemia |
| Study Type | Experimental human endotoxemia study |
| Study Summary | Study to investigate the effects of a training program encompassing cold exposure, breathing exercises, and meditation on plasma metabolomic during experimental human endotoxemia. |
| Institute | Radboud University Medical Centre |
| Department | Intensive Care Medicine |
| Laboratory | Metabolomic Discoveries (acquired by Metabolon in September 2017) |
| Last Name | Kox |
| First Name | Matthijs |
| Address | Intensive Care Medicine (710), Geert Grooteplein 10 |
| matthijs.kox@radboudumc.nl | |
| Phone | 243653881 |
| Submit Date | 2019-09-16 |
| Num Groups | 2 |
| Total Subjects | 24 |
| Num Males | 24 |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-10-04 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001327 |
| Sampleprep Summary: | Sample extraction was performed as described previously (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154597), with some modifications. Briefly, 50 µL of plasma was mixed with 450 µL of 90% (v/v) methanol containing internal standards and incubated for 15 min at 37°C with 1150 rpm. Precipitated proteins were separated from the extract by centrifugation for 12 min at 15000 rpm, after which supernatants were stored at -80 °C until further analysis. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Ethanol precipitation |
