Summary of Study ST001316
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000893. The data can be accessed directly via it's Project DOI: 10.21228/M8869V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001316 |
Study Title | Time-course experiment of Microchloropsis gaditana cells supplemented with CO2 |
Study Type | Time-course experiment |
Study Summary | Experiments were conducted with Microchloropsis gaditana supplemented with very-low CO2 and high CO2. Sampling was done on the following time points: Day 3, 6 and 9. |
Institute | International Centre for Genetic Engineering and Biotechnology |
Department | Integrative Biology |
Laboratory | Omics of Algae |
Last Name | Jutur |
First Name | Pannaga Pavan |
Address | 2nd Floor, New Building, ICGEB, Aruna Asaf Ali Marg, New Delhi - 110067 |
jppavan@icgeb.res.in | |
Phone | +91 11 26741358 |
Submit Date | 2020-02-04 |
Study Comments | Former name of species: Nannochloropsis gaditana Lubián |
Raw Data Available | Yes |
Raw Data File Type(s) | mzdata.xml |
Analysis Type Detail | LC-MS |
Release Date | 2021-02-05 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001398 |
Sampleprep Summary: | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. |