Summary of Study ST001316

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000893. The data can be accessed directly via it's Project DOI: 10.21228/M8869V This work is supported by NIH grant, U2C- DK119886.

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Study IDST001316
Study TitleTime-course experiment of Microchloropsis gaditana cells supplemented with CO2
Study TypeTime-course experiment
Study SummaryExperiments were conducted with Microchloropsis gaditana supplemented with very-low CO2 and high CO2. Sampling was done on the following time points: Day 3, 6 and 9.
Institute
International Centre for Genetic Engineering and Biotechnology
DepartmentIntegrative Biology
LaboratoryOmics of Algae
Last NameJutur
First NamePannaga Pavan
Address2nd Floor, New Building, ICGEB, Aruna Asaf Ali Marg, New Delhi - 110067
Emailjppavan@icgeb.res.in
Phone+91 11 26741358
Submit Date2020-02-04
Study CommentsFormer name of species: Nannochloropsis gaditana Lubián
Raw Data AvailableYes
Raw Data File Type(s)mzdata.xml
Analysis Type DetailLC-MS
Release Date2021-02-05
Release Version1
Pannaga Pavan Jutur Pannaga Pavan Jutur
https://dx.doi.org/10.21228/M8869V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001398
Sampleprep Summary:Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis.
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