Summary of Study ST001320
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000897. The data can be accessed directly via it's Project DOI: 10.21228/M8R704 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001320 |
| Study Title | Examination of labeled glucose utilization in central carbon metabolism in HeLa cells post WNT stimulation |
| Study Type | In vitro |
| Study Summary | HeLa cells were treated with media containing either vehicle or WNT in combination with UC13-glucose for 20 min or 60 min. |
| Institute | University of California, Los Angeles |
| Department | Biological Chemisty |
| Laboratory | Christofk Lab |
| Last Name | Christofk |
| First Name | Heather |
| Address | 615 Charles E Young Drive South Los Angeles, CA 90095 |
| hchristofk@mednet.ucla.edu | |
| Phone | (310) 794-4248 |
| Submit Date | 2020-02-19 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-07-18 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001402 |
| Sampleprep Summary: | Extraction began by placing the 6-well dishes on ice and washing each well with 2mL of a 150 mM ammonium acetate solution (pH 7.4, 4°C). Post wash, metabolites were extracted from each well on dry ice with 500 μL of a (1:4) dH20, MeOH solution at -80°C. The samples were then incubated for 15 min at -80°C. Afterwards, the metabolite solutions were transferred to eppitubes and spun down at 4°C for 10 min at 17,000g. The supernatants were transferred to new vials and dried with an EZ2-Elite lyophilizer (Genevac). These dried metabolites were then stored at -80°C until they could be run on the LC/MS. |
| Extract Storage: | -80℃ |
