Summary of Study ST001324

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000901. The data can be accessed directly via it's Project DOI: 10.21228/M8770G This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001324
Study TitleMetabolomics Adaptation of Juvenile Pacific Abalone Haliotis discus hannai to Heat Stress
Study SummaryWe compared two groups of abalones (from the same population) with different temperature acclimation history, through their survival at acute heat treatment and metabolites changes. The results indicated significantly higher survival for the high temperature acclimation group. Both groups experienced mitochondrial homeostasis break down during heat treatment, while the higher temperature acclimation group accumulated more metabolites beneficial to metabolic homeostasis, including various dipeptides, antioxidants, and neuroprotective substances.
Institute of Oceanology, Chinese Academy of Sciences
Last NameXu
First NameFei
Address7 Nanhai Road, Qingdao, Shandong, 266071, China
Submit Date2020-01-30
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2020-09-10
Release Version1
Fei Xu Fei Xu application/zip

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Sample Preparation:

Sampleprep ID:SP001406
Sampleprep Summary:50 mg of sample was taken and placed in an Eppendorf tube, followed by the addition of 1000 µl extraction solvent containing an internal target (V methanol : V acetonitrile : V water = 2 : 2 : 1, containing internal standard 2 µg/ml). The mixture was homogenized in ball mill for 4 min at 45 Hz, then ultrasound treated for 5min (incubated in ice water). After homogenization for 3 times, the mixture was incubated for 1 h at -20 °C to precipitate proteins. Centrifugation was performed at 12000 rpm for 15 min at 4 °C. A total of 825 µl supernatant was carefully transferred into a fresh Eppendorf tube, and dried in a vacuum concentrator without heating. 200 µl extraction solvent (V acetonitrile: V water = 1:1) was then added for reconstitution, followed by vortex 30 s and sonicated for 10 min (4 °C water bath), centrifuge for 15 min at 12000 rpm (4 °C). 75 µl supernatant was transferred into a fresh 2 ml LC/MS glass vial for UHPLC-QTOF-MS analysis, while another 20 µl was taken from each sample and pooled as QC samples. Six technical replicates were conducted to QC sample with 75 µL each for UHPLC-QTOF-MS analysis.
Sampleprep Protocol Filename:LC-MS-Q211.pdf