Summary of Study ST001350

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000920. The data can be accessed directly via it's Project DOI: 10.21228/M8S11R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001350
Study Title	Extraction of high-resolution Metabolomics data of the Yeast Metabolic Cycle
Study Summary	Extraction of a high resolution metabolomics dataset through the Yeast Metabolic Cycle to complete a multi-omics dataset in this model system
Institute	University of Florida
Last Name	Conesa
First Name	Ana
Address	2033 Mowry Rd, Gainesville, FL 32610-3633
Email	salvacasani@gmail.com / aconesa@ufl.edu
Phone	352-273-8127
Submit Date	2020-03-06
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2020-12-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001432
Sampleprep Summary:	Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC.

Sampleprep ID:

SP001432

Sampleprep Summary:

Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC.