Summary of Study ST001380
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000944. The data can be accessed directly via it's Project DOI: 10.21228/M8P41V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001380 |
Study Title | Fast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions |
Study Summary | Mass spectrometry based metabolomics is a widely used approach in biotechnology and biomedical research. However, current methods coupling mass spectrometry with chromatography are time-consuming and not suitable for high-throughput analysis of thousands of samples. An alternative approach is flow-injection mass spectrometry (FI-MS) in which samples are directly injected to the ionization source. Here, we show that the sensitivity of Orbitrap FI-MS metabolomics methods is limited by ion competition effect in the detection system. We describe an approach for overcoming this effect by analyzing the distribution of ion m/z values and computationally determining a series of optimal scan ranges. This enables reproducible detection of ~9,000 and ~10,000 m/z features in metabolomics and lipidomics analysis of serum samples, respectively, with a sample scan time of ~15 seconds and duty time of ~30 seconds; a ~50% increase versus current spectral-stitching FI-MS. This approach facilitates high-throughput metabolomics for a variety of applications, including biomarker discovery and functional genomics screens. |
Institute | Technion – Israel Institute of Technology |
Last Name | Shlomi |
First Name | Tomer |
Address | Technion |
tomersh@cs.technion.ac.il | |
Phone | +972-77-8871497 |
Submit Date | 2020-05-17 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML, raw(Thermo) |
Analysis Type Detail | MS(Dir. Inf.) |
Release Date | 2020-05-22 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001462 |
Sampleprep Summary: | To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis. |