Summary of study ST001380

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000944. The data can be accessed directly via it's Project DOI: 10.21228/M8P41V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001380
Study TitleFast and sensitive flow-injection mass spectrometry metabolomics by analyzing sample specific ion distributions
Study SummaryMass spectrometry based metabolomics is a widely used approach in biotechnology and biomedical research. However, current methods coupling mass spectrometry with chromatography are time-consuming and not suitable for high-throughput analysis of thousands of samples. An alternative approach is flow-injection mass spectrometry (FI-MS) in which samples are directly injected to the ionization source. Here, we show that the sensitivity of Orbitrap FI-MS metabolomics methods is limited by ion competition effect in the detection system. We describe an approach for overcoming this effect by analyzing the distribution of ion m/z values and computationally determining a series of optimal scan ranges. This enables reproducible detection of ~9,000 and ~10,000 m/z features in metabolomics and lipidomics analysis of serum samples, respectively, with a sample scan time of ~15 seconds and duty time of ~30 seconds; a ~50% increase versus current spectral-stitching FI-MS. This approach facilitates high-throughput metabolomics for a variety of applications, including biomarker discovery and functional genomics screens.
Institute
Technion – Israel Institute of Technology
Last NameShlomi
First NameTomer
AddressTechnion
Emailtomersh@cs.technion.ac.il
Phone+972-77-8871497
Submit Date2020-05-17
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2020-05-22
Release Version1
Tomer Shlomi Tomer Shlomi
https://dx.doi.org/10.21228/M8P41V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001462
Sampleprep Summary:To extract metabolites and lipids from serum samples, we mixed 20 µL of serum with an extraction solution for metabolomics analysis and 10 µL for lipidomics in 96-deep well plates. For lipidomics analysis, we utilized 100 µL of 2-propanol/methanol (6:1, v/v); and for metabolomics analysis, 100 µL of methanol/acetonitrile/water (5:3:1, v/v/v). After 10 min of vortexing, 800 rpm, precipitated proteins were separated by centrifugation for 20 min at 4 °C and 4000 rcf; supernatants were stored at -80 °C prior the analysis.
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