Summary of Study ST001384

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000948. The data can be accessed directly via it's Project DOI: 10.21228/M8540T This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001384
Study TitlePlasmodium falciparum increased time in circulation underlies persistent asymptomatic infection in the dry season
Study SummaryThe dry season is a major challenge for Plasmodium falciparum parasites in many malaria endemic regions, where water availability limits mosquitoes to only part of the year. How P. falciparum bridges two transmission seasons months apart, without being cleared by the host or compromising host survival is poorly understood. Here we show that low levels of P. falciparum parasites persist in the blood of asymptomatic Malian individuals during the 5- to 6-month dry season, rarely causing symptoms and minimally affecting the host immune response. Parasites isolated during the dry season are transcriptionally distinct from those of subjects with febrile malaria in the transmission season, reflecting longer circulation within each replicative cycle, of parasitized erythrocytes without adhering to the vascular endothelium. Low parasite levels during the dry season are not due to impaired replication, but rather increased splenic clearance of longer-circulating infected erythrocytes. We propose that P. falciparum virulence in areas of seasonal malaria transmission is regulated so that the parasite decreases its endothelial binding capacity, allowing increased splenic clearance and enabling several months of subclinical parasite persistence.
Institute
Pennsylvania State University
Last NameLlinas
First NameManuel
AddressW126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA
Emailmanuel@psu.edu
Phone(814) 867-3527
Submit Date2020-05-25
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-08-20
Release Version1
Manuel Llinas Manuel Llinas
https://dx.doi.org/10.21228/M8540T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001466
Sampleprep Summary:Each plasma sample was split into two independent samples for metabolite extraction. For hydrophilic metabolites, 50µL of plasma was extracted by the addition of 9X volumes of ice cold methanol. Samples were briefly vortexed before centrifuging for 10 minutes to remove precipitated protein. The clarified supernatants were dried under nitrogen gas and resuspended in 100µL (1:2 dilution final). For hydrophobic metabolites, 25µL of plasma was extracted by the addition of 3X volumes of isopropanol. Samples were briefly vortexed and allowed to sit at room temperature for 10 minutes. Samples were then placed at -20 °C to precipitate overnight. Precipitated samples were centrifuged for 20 minutes and the clarified supernatant was diluted to 50% water in a glass LCMS sample vial (1:6 dilution final). Sample groups were pooled to create a group QA and all samples were pooled to create a batch QC, which were injected periodically throughout each run.
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