Summary of Study ST001399

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000959. The data can be accessed directly via it's Project DOI: 10.21228/M8QX3V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001399
Study Title	Activation of ectopic olfactory receptor 544 induces GLP-1 secretion, alters gut microbiome, and improves intestinal permeability.
Study Type	Fecal metabolome
Study Summary	Metabolome data set from mouse fecal samples Group - WT_AZA: fecal samples from wild type mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - WT_DW: fecal samples from wild type mouse fed with high fat diet Group - KO_AZA: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet and azelaic acid (0.05%, w/w) Group - KO_DW: fecal samples from Olfr544 receptor knock out mouse fed with high fat diet
Institute	Korea University
Last Name	Kim
First Name	Jungyeon
Address	145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South
Email	kim131812@korea.ac.kr
Phone	821082248015
Submit Date	2020-06-07
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2020-06-30
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001481
Sampleprep Summary:	Methoximation and silylation were performed for qualification and relative quantification of metabolites using GC/TOF–MS, For methoximation, 10 µL of 40 mg/mL methoxyamine hydrochloride in pyridine (Sigma-Aldrich) was added to the dried sample, and the mixture was incubated at 30°C for 90 minutes. For silylation, 45 µL of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) was added to the mixture, and the mixture was incubated at 37°C for 30 minutes.

Sampleprep ID:

SP001481

Sampleprep Summary:

Methoximation and silylation were performed for qualification and relative quantification of metabolites using GC/TOF–MS, For methoximation, 10 µL of 40 mg/mL methoxyamine hydrochloride in pyridine (Sigma-Aldrich) was added to the dried sample, and the mixture was incubated at 30°C for 90 minutes. For silylation, 45 µL of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) was added to the mixture, and the mixture was incubated at 37°C for 30 minutes.