Summary of Study ST001448

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000995. The data can be accessed directly via it's Project DOI: 10.21228/M8311J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001448
Study Title	Maternal Hypercortisolemia alters placental metabolism: NMR metabolomics
Study Summary	NMR metabolomic studies of placental tissue from sheep with excess maternal cortisol during late gestation
Institute	University of Florida
Last Name	Walejko
First Name	Jacquelyn
Address	300 N Duke St Durham NC 27701
Email	jacquelyn.walejko@duke.edu
Phone	9194792304
Submit Date	2020-08-18
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2020-09-16
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001530
Sampleprep Summary:	High-resolution magic angle spinning (HR-MAS) proton nuclear magnetic resonance (1H-NMR) was used to determine metabolites present in placental tissue. Briefly, 30 μL of deuterium oxide (D2O) with 3.3 mM 3-(Trimethylsilyl)-1-propanesulfonic acid (DSS) as a reference standard was added to 27.7-52.1 mg (mean 35.3 mg) of placental tissue before being placed into a 4 mm HR-MAS rotor (Bruker Biospin) and spun at 6 kHz with a spectral width of 10 ppm. Data were acquired on an Avance III 600 MHz Bruker NMR spectrometer equipped with a 4 mm HR-MAS probe at the University of Georgia Complex Carbohydrate Research Center. Data were acquired using a one-dimensional experiment with T2 filter using Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with water presaturation (CPMGPR1D).

Sampleprep ID:

SP001530

Sampleprep Summary:

High-resolution magic angle spinning (HR-MAS) proton nuclear magnetic resonance (1H-NMR) was used to determine metabolites present in placental tissue. Briefly, 30 μL of deuterium oxide (D2O) with 3.3 mM 3-(Trimethylsilyl)-1-propanesulfonic acid (DSS) as a reference standard was added to 27.7-52.1 mg (mean 35.3 mg) of placental tissue before being placed into a 4 mm HR-MAS rotor (Bruker Biospin) and spun at 6 kHz with a spectral width of 10 ppm. Data were acquired on an Avance III 600 MHz Bruker NMR spectrometer equipped with a 4 mm HR-MAS probe at the University of Georgia Complex Carbohydrate Research Center. Data were acquired using a one-dimensional experiment with T2 filter using Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with water presaturation (CPMGPR1D).