Summary of Study ST001497

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001014. The data can be accessed directly via it's Project DOI: 10.21228/M8MT3W This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001497
Study Title	Intraday validation of of developed quantatation method
Study Summary	To validate this method , we spiked either taurine or sulfoquinovose into fecal supernatents and determined intraday accuracy and precission of method
Institute	Helmholtz Centre for Environmental Research
Last Name	Haange
First Name	Sven
Address	Permoserstrasse 1, Leipzig, Saxony, 04318, Germany
Email	sven.haange@ufz.de
Phone	0049 341 2351099
Submit Date	2020-10-02
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2020-10-28
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001579
Sampleprep Summary:	Fecal supernatants were serially diluted using 50% acetonitrile and 0.1% formic acid in water to obtain final dilution of 1:10,000. 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis.For the analysis of sulfonates by LC-MS/MS-MRM, aliquots of 250 µl for each sample were centrifuged (14 000 x g, 4 °C, 5 min) and 50 µl of the supernatant stored at – 20 °C until further processing. Samples were thawed, centrifuged at 18 000 x g at RT for 2 min and the supernatant was diluted 1:10 000 in 50% aqueous acetonitrile. Subsequently, 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis.
Sampleprep Protocol Filename:	sven_haange_Sample_preparation-application_experiment.pdf