Summary of Study ST001525
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001026. The data can be accessed directly via it's Project DOI: 10.21228/M82T3X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001525 |
Study Title | Perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) alter the blood lipidome and the hepatic proteome in a murine model of diet-induced obesity |
Study Summary | Perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) alter the blood lipidome and the hepatic proteome in a murine model of diet-induced obesity |
Institute | University of Rhode Island;University of Georgia |
Department | Pharmaceutical and Biomedical Sciences |
Laboratory | Cummings/Slitt |
Last Name | Ingram |
First Name | Lishann |
Address | 250 West Green Street Athens, GA 30605 |
ingram@carnegiescience.edu | |
Phone | 706-542-3792 |
Submit Date | 2020-07-30 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001607 |
Sampleprep Summary: | Blood lipids were isolated for lipidomic analysis according to the Bligh and Dyer method (Bligh and Dyer 1959). The lipidomics was performed at the University of Georgia (Athens, GA). Briefly, blood samples designated for lipidomics were suspended in 1.25 ml of methanol and 1.25 ml of chloroform. Tubes were vortexed for 30 s, allowed to sit for 10 min on ice, centrifuged (300 x g; 5 min), and the bottom chloroform layer was transferred to a new test tube. The extraction steps were repeated three times and the chloroform layer combined. A commercial mix of SPLASH Lipidomix internal standards (Avanti Polar Lipids, Inc.) were spiked into each sample. SPLASH Lipidomix Mass Spec standards includes all major lipid classes at ratios similar to that found in human plasma. The collected chloroform layers were dried under nitrogen, reconstituted with 50 µl of methanol: chloroform (3:1 v/v), and stored at 80ºC until analysis. Lipid content was quantified by determining the level of inorganic phosphorus using the Bartlett Assay (Bartlett 1959). |