Summary of Study ST001634

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001045. The data can be accessed directly via it's Project DOI: 10.21228/M8MQ3V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001634
Study TitleA combinatorial action of GmMYB176 and bZIP controls isoflavonoid biosynthesis in soybean.
Study SummaryThis study identified how the GmMYB176 protein complex affects the metabolome of soybean hairy roots using non-targeted high resolution mass spectrometry.
Institute
Agriculture and Agri-Food Canada
Last NameRenaud
First NameJustin
Address1391 Sandford Street, London, Ontario, N5V 4T3, Canada
Emailjustin.renaud@canada.ca
Phone519-953-6698
Submit Date2020-12-17
Num Groups2
Total Subjects10
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-01-07
Release Version1
Justin Renaud Justin Renaud
https://dx.doi.org/10.21228/M8MQ3V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001717
Sampleprep Summary:For metabolomics analysis, frozen hairy roots were ground with liquid nitrogen and extracted in methanol:water (80:20, v/v). The samples were sonicated on ice water bath for 15 min followed by centrifugation at 11,000×g for 10 min at ambient temperature. The supernatant (350 µL) was dried under nitrogen gas. The dried pellet was dissolved in 200 µL of 50% methanol containing 10 µg caffeine as an internal standard and filtered through a 0.45 µm syringe filter.
Sampleprep Protocol ID:jrenaud_SP_Sample_preparation.pdf
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