Summary of Study ST001680

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001079. The data can be accessed directly via it's Project DOI: 10.21228/M8797T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST001680
Study Title	Metabolome of NAFLD in high fat diet mouse model
Study Summary	We investigated the effects of a RAR beta 2 agonist, AC261066, on the hepatic metabolites changed in high fat fed NAFLD mouse model. We suggest that AC261066 has potential therapeutic relevance for the prevention/treatment of NAFLD and NASH.
Institute	Weill Cornell Medicine
Last Name	Gudas
First Name	Lorraine
Address	1300 York Ave
Email	chl2029@med.cornell.edu
Phone	646-3094389
Submit Date	2021-01-27
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2022-01-27
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001763
Sampleprep Summary:	The snap frozen liver samples were incubated with 600 µL of 3 mM monobrombimane (MBB) in CH3OH:H2O (80:20) at -20°C for 2 h, followed by 1 h incubation at 0°C. This initial incubation was followed by tissue disruption using stainless steel beads in a TissueLyser (Qiagen) and an additional 30 min at -20°C. Extracts were centrifuged for 15 min at 13000 rpm to pellet insoluble material and supernatants were transferred to clean tubes. This extraction was repeated two additional times and all three supernatants were dried in a speed-vac (Savant) and stored at −80 °C until analysis.

Sampleprep ID:

SP001763

Sampleprep Summary:

The snap frozen liver samples were incubated with 600 µL of 3 mM monobrombimane (MBB) in CH3OH:H2O (80:20) at -20°C for 2 h, followed by 1 h incubation at 0°C. This initial incubation was followed by tissue disruption using stainless steel beads in a TissueLyser (Qiagen) and an additional 30 min at -20°C. Extracts were centrifuged for 15 min at 13000 rpm to pellet insoluble material and supernatants were transferred to clean tubes. This extraction was repeated two additional times and all three supernatants were dried in a speed-vac (Savant) and stored at −80 °C until analysis.