Summary of Study ST001684

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001083. The data can be accessed directly via it's Project DOI: 10.21228/M8Q98J This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001684
Study Title	An overexpression of lipoprotein lipase leads to an alteration in the skeletal muscle metabolome in transgenic rabbits
Study Summary	The purpose of the current study was to characterize the skeletal muscle metabolome in the lipoprotein lipase (LPL) transgenic rabbits. The skeletal muscle metabolite profile was analyzed using capillary electrophoresis time-of flight mass spectrometry in the control rabbits and LPL transgenic rabbits (n = 9, each group).
Institute	Saga University
Last Name	Nishida
First Name	Yuichiro
Address	Department of Preventive Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501
Email	ynishida@cc.saga-u.ac.jp
Phone	+81 952 34 2287
Submit Date	2020-12-03
Analysis Type Detail	LC-MS
Release Date	2022-01-02
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001767
Sampleprep Summary:	The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement.

Sampleprep ID:

SP001767

Sampleprep Summary:

The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement.