Summary of Study ST001789

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001133. The data can be accessed directly via it's Project DOI: 10.21228/M87X24 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001789
Study TitleAcute metabolomic changes of plasma in response to endurance exercise
Study SummaryThis study compared the metabolomic differences in plasma of young adult volunteers between before (Pre), immediately after the run (Time 0) and 60 minutes after the 90-min run (Time 60).
University of California, San Diego
DepartmentDepartment of Medicine
LaboratoryThe Naviaux Lab
Last NameNaviaux
First NameRobert
Address214 Dickinson St., Room C-107
Submit Date2021-04-21
Num Groups3
Total Subjects19
Num Males13
Num Females6
Analysis Type DetailLC-MS
Release Date2022-01-06
Release Version1
Robert Naviaux Robert Naviaux application/zip

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Sample Preparation:

Sampleprep ID:SP001872
Sampleprep Summary:Plasma samples (90 µl) were mixed with 5 µl of commercial internal standards and 5 µl of custom-synthesized 13C labeled standards and then incubated for 10 min at room temperature to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. The metabolites were extracted with 400 µl of cold (-20 °C) methanol: acetonitrile (50:50, v/v), yielding a final concentration of methanol: acetonitrile: water (40:40:20, v/v/v). The samples were then vortexed vigorously, incubated on crushed ice for 10 min, and then centrifuged at 16,000 g for 10 min at 4 °C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80 °C prior to LC-MS/MS.
Processing Storage Conditions:On ice
Extract Storage:-80℃