Summary of Study ST001829

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001156. The data can be accessed directly via it's Project DOI: 10.21228/M88T3S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001829
Study TitleAnalysis and annotation of oxidized PCs generated in vitro.
Study SummaryOxidized PC16:0/PUFA (18:2, 20:4 and 22:6) generated in vitro were analyzed by LC/HRMS/MS analysis.All mass spectrometry raw data obtained in this study were deposited.
Institute
Kyushu university
Last NameMatsuoka
First NameYuta
Address3-1-1 Maidashi Higashi-ku
Emailymatsu1205@gmail.com
Phone+81-92-642-6624
Submit Date2021-06-13
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-10-14
Release Version1
Yuta Matsuoka Yuta Matsuoka
https://dx.doi.org/10.21228/M88T3S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001912
Sampleprep Summary:For in vitro experiments, the reaction solutions were extracted using the modified Bligh and Dyer method. Briefly, a mixed solution of 1 mL methanol and 1 mL chloroform, containing 100 μM dibutylhydroxytoluene and ethylenediaminetetraacetic acid as antioxidants, and 100 nM PC15:0/18:1-d7 as an internal standard was added to the reaction solution. After 1 min vortex, the organic layer was collected in a glass tube. The extracted solution was dried under a stream of nitrogen gas, and the residue was dissolved in methanol (200 µL) and stored at −80 °C before injection into the HPLC column.
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