Summary of Study ST001830
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001156. The data can be accessed directly via it's Project DOI: 10.21228/M88T3S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001830 |
Study Title | Exhaustive analysis of endogenous oxPCs in APAP-treated mice. |
Study Summary | A single dose of APAP at 300 mg/kg body weight was intraperitoneally administered into eight-week-old C57BL/6J male mice. After extraction of hepatic lipids, oxPCs were analyzed by LC/HRMS/MS. All mass spectrometry raw data obtained in this study were deposited. |
Institute | Kyushu university |
Last Name | Matsuoka |
First Name | Yuta |
Address | 3-1-1 Maidashi Higashi-ku, Fukuoka, Not USCanada, 812-8582, Japan |
ymatsu1205@gmail.com | |
Phone | +81-92-642-6624 |
Submit Date | 2021-06-13 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2021-10-14 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001913 |
Sampleprep Summary: | Hepatic lipids were extracted from the liver samples according to the modified Bligh and Dyer method. Briefly, 1 mL of extraction solution (methanol:chloroform:water = 5:2:2) containing 100 μM dibutylhydroxytoluene, 100 μM ethylenediaminetetraacetic acid, and 100 nM PC15:0/18:1-d7 was added to a frozen tissue sample (wet weight: approx. 50 mg), and then, the sample was homogenized using a Macro Smash homogenizer. Subsequently, the extraction solutions were sonicated on ice bath for 5 min. After centrifugation (6,000 g, 10 min, 4°C), 700 µL of the supernatant was collected, and then, 235 µL chloroform and 155 µL water were added to the supernatant. The organic layer was collected in a glass tube and dried under a stream of nitrogen gas; the dried residue was dissolved in methanol (200 µL) and stored at −80 °C before performing the LC/HRMS/MS experiments. |