Summary of Study ST001860

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001173. The data can be accessed directly via it's Project DOI: 10.21228/M8341K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001860
Study TitleSpontaneous hydrolysis and spurious metabolic properties of α-ketoglutarate esters
Study TypeManuscript
Study Summary8988T cells treated with methyl acetate or 1 mM of alpha-ketoglutarate disodium salt or 1 mM of dimethyl-alpha-ketoglutarate for 3 hours prior to rapid quenching of metabolism and extraction of metabolites in 80% methanol (-80°C) containing internal QC standards.
Institute
University of British Columbia
Last NameParker
First NameSeth
Address950 W 28th Ave, 2099, Vancouver, British Columbia, Canada V6H 0B3
Emailseth.parker@bcchr.ca
Phone6048753121
Submit Date2021-05-26
Num Groups3
Total Subjects9
Num Malesn/a
Num Femalesn/a
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2021-08-04
Release Version1
Seth Parker Seth Parker
https://dx.doi.org/10.21228/M8341K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001943
Sampleprep Summary:Dried samples were reconstituted in 50 µL of Optima LC/MS grade water (Fisher Scientific, Waltham, MA). Samples were sonicated for 2 mins, then centrifuged at 21,000 x g for 3 min at 4°C. Twenty microliters were transferred to LC vials containing glass inserts for analysis. The remaining sample was placed in -80°C for long term storage.
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