Summary of Study ST001897
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001193. The data can be accessed directly via it's Project DOI: 10.21228/M8H419 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001897 |
| Study Title | A local source of insulin in the eye governed by phagocytosis and starvation |
| Study Type | Untargeted Metabolomics |
| Study Summary | Untargeted metabolite analysis was performed on a Thermo Orbitrap IDX Tribrid MS to understand changes in metabolites in eye retina and RPE tissue during starvation. This study also probes the role of certain metabolites during phagocytosis. |
| Institute | University of Virginia |
| Department | Center of Cell Clearance , Microbiology, Immunology, and Cancer Biology |
| Laboratory | Kodi Ravichandraan Lab |
| Last Name | Etchegaray |
| First Name | Iker |
| Address | Center of Cell Clearance , Microbiology, Immunology, and Cancer Biology |
| kr4h@virginia.edu; jie3c@virginia.edu; nw5es@virginia.edu; | |
| Phone | 4023109931 |
| Submit Date | 2021-08-02 |
| Num Groups | 4 |
| Total Subjects | 2 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001981 |
| Sampleprep Summary: | Overnight fasted mice were euthanized with avertin after light onset. Five replicates each of both Retinal and RPE tissues (from wild type and knock out mice) were harvested/dissected, flash frozen and stored in -80 ºC until extraction. Tissues were thawed on ice and extracted using methanol:chloroform (2:1) at 4C and phase separated using water. Top aq. phase was recovered as metabolite mixture and dried overnight in speedVac and reconstituted in 100 µL of 0.1% Formic acid in water. Ten µl from each tube was removed to create a pooled QC sample that was injected at the beginning and end of the MS sequence run and additional QC samples were injected after every 5 sample injection. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
