Summary of Study ST001907
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001201. The data can be accessed directly via it's Project DOI: 10.21228/M8G69Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001907 |
| Study Title | Training-induced bioenergetic improvement in human skeletal muscle is associated with non-stoichiometric changes in the mitochondrial proteome without reorganisation of respiratory chain content |
| Study Type | Multi Omics |
| Study Summary | Lipidomic analysis of muscle mitochondrial isolates. 10 men with repeated measures. |
| Institute | Baker Heart and Diabetes Institute |
| Department | Meteabolomics |
| Laboratory | Meteabolomics |
| Last Name | Huynh |
| First Name | Kevin |
| Address | 75 Commercial Road |
| kevin.huynh@baker.edu.au | |
| Phone | 0385321537 |
| Submit Date | 2021-08-15 |
| Num Groups | 1 |
| Total Subjects | 10 |
| Num Males | 10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-10-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001991 |
| Sampleprep Summary: | Mitochondrial isolates were extracted using a modified single-phase chloroform/methanol extraction as described previously (Weir et al. 2013). In brief, 20 volumes of chloroform:methanol (2:1) were added to the sample along with a series of internal standards. Samples were vortexed and centrifuged on a rotary mixer for 10 min. Following sonication on a sonicator bath for 30 min, samples were rested for 20 min prior to centrifugation at 13,000 g for 10 min. Supernatants were transferred into a 96 well plated, dried down and reconstituted in 50 μL H2O saturated butanol and sonicated for 10 min. After the addition of 50 μL of methanol with 10 mM ammonium 945 formate, samples were centrifuged at 4000 rpm on a plate centrifuge and transferred into glass vials with inserts for mass spectrometry analysis. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Single phase 2:1 chloroform methanol |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Butanol : Methanol 1:1 |
| Subcellular Location: | Mitochondria |
