Summary of Study ST001927

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001217. The data can be accessed directly via it's Project DOI: 10.21228/M8D693 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001927
Study TitleFungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach.
Study TypeUntargeted Metabolomics
Study SummaryEntomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previosly.
Universidade Federal do Paraná
DepartmentPatologia Básica
LaboratoryLaboratório de Microbiologia e Biologia Molecular
Last NameStuart
First NameAndressa
AddressAv. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil
Submit Date2021-09-29
Num Groups3
Total Subjects15
Study CommentsTwo genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control.
Raw Data AvailableYes
Raw Data File Type(s)cdf, raw(Waters)
Analysis Type DetailGC/LC-MS
Release Date2022-10-03
Release Version1
Andressa Stuart Andressa Stuart application/zip

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Sample Preparation:

Sampleprep ID:SP002011
Sampleprep Summary:Extraction was performed in microtubes, from 200 mg of fungal macerate to which 1 mL of 6:2:2 methanol:chloroform:water ice-cold extraction solution was added. These extraction microtubes were vigorously vortexed and placed in an ultrasonic low-temperature bath at 20 Hz s-1 for 15 min. The samples were then centrifuged (Eppendorf, Germany) at 4°C for 10 min at 14,000 rpm. Then, the supernatant was filtered using a 0.22 μm Whatman® filter (Merck, Germany) and transferred to a chromatographic vial where the extracts were lyophilized (Thermo Fischer Scientific, MA, USA) until completely dry. Finally, the lyophilized samples were resuspended in 200 μL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS.
Processing Storage Conditions:-80℃
Extract Storage:-80℃