Summary of Study ST002016
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001280. The data can be accessed directly via it's Project DOI: 10.21228/M88715 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002016 |
| Study Title | Metabolomics of COVID patients |
| Study Type | Untargeted Metabolomics |
| Study Summary | Untargeted metabolite analysis was performed on a Thermo Orbitrap IDX Tribrid MS to understand changes in metabolites due to COVID severity. |
| Institute | University of Virginia |
| Department | 1Department of Biochemistry & Molecular Genetics; School of Medicine Core Facilities; Department of Microbiology, Immunology, and Cancer Biology; Department of Biomedical Engineering |
| Laboratory | Biomolecular Analysis Facility, Univ of Virginia School of Medicine |
| Last Name | Wase |
| First Name | Nishikant |
| Address | Biomolecular Analysis Facility, Pinn Hall Room No 1105B |
| nw5es@virginia.edu | |
| Phone | 4023109931 |
| Submit Date | 2021-12-14 |
| Num Groups | 6 |
| Total Subjects | 140 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-07-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002103 |
| Sampleprep Summary: | plasma samples were thawed on ice and 50 µL of plasma was retained for the metabolome analysis. Approximately 200 µL of cold methanol was added to the plasma sample and shaken vigorously to inactivate any potential viruses. The samples were stored in -80 ºC immediately until extraction for metabolomics experiments. For extraction, 200 µL of cold methanol was added to each tube, vortexed and shaken vigorously for 30 min at 4 ºC in a temperature controlled thermal shaker. Further 200 µL of chloroform and 400 µL of water were added, shaken vigorously and the top aqueous phase was recovered as a metabolite mixture of diverse chemical nature. Each metabolite extract was dried overnight in speedVac and reconstituted in 60 µL of 0.1% formic acid in water. Ten microliters from each tube were removed to create a pooled QC sample that was injected at the beginning and end of the MS sequence run and additional QC samples were injected after every 10 sample injections. |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | Described above |
| Extract Storage: | Described in summary |
| Sample Resuspension: | 0.1% formic acid |
