Summary of Study ST002023

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001285. The data can be accessed directly via it's Project DOI: 10.21228/M8MD8N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002023
Study Title	A targeted metabolomics study for assessing rodent thyroid toxicity
Study Summary	The thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.
Institute	Helmholtz Centre for Environmental Research
Department	Department of Molecular Systems biology
Laboratory	Functional Metabolomics
Last Name	Wang
First Name	Zhipeng
Address	Permoserstraße 15, Leipzig, Saxony, 04318, Germany
Email	zhipeng.wang@ufz.de
Phone	+49 341 235 1078
Submit Date	2021-12-20
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2022-12-20
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002111
Sampleprep Summary:	Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.

Sampleprep ID:

SP002111

Sampleprep Summary:

Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.