Summary of Study ST002236
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001426. The data can be accessed directly via it's Project DOI: 10.21228/M8D42R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002236 |
| Study Title | The impact of IgE in gut and serum metabolomes in a murine experimental model of allergic enteritis |
| Study Type | Case-control study |
| Study Summary | The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma, and anaphylaxis, partly due to difficulty in the access to intestinal tissues and because of a highly complex interplay between microbiota and intestinal mucosa. Importantly, a high level of IgE is a poor prognostic factor in gastrointestinal allergies. This study aimed to investigate how IgE influences the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. Ovalbumin-sensitized and egg-white diet fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulation. |
| Institute | Institute of Applied Molecular Medicine |
| Last Name | Zubeldia-Varela |
| First Name | Elisa |
| Address | Avda. Monteprincipe s/n 28668 Boadilla del Monte, Madrid, España |
| elisa.zubeldiavarela@ceu.es | |
| Phone | Tlf: 91 372 47 00 ext. 14675 |
| Submit Date | 2022-07-19 |
| Num Groups | 4 groups: BALB/c wild type (WT) and IgE knock-in mice (C.Ighg1tm1.1Pyu) in the BALB/c background, both sensitised and non-sensitised to ovalbumin. |
| Total Subjects | 29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC/LC-MS |
| Release Date | 2022-08-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002328 |
| Sampleprep Summary: | FAECES. Frozen faeces samples were lyophilized overnight at -80°C. Faecal pellets were doubly extracted (i) first with MeOH:MTBE (4:1, v/v) and (ii) with MeOH:H2O (4:1, v/v). Equal volumes (300 µL) of the supernatants from (i) and (ii) were then mixed and aliquoted for subsequent analysis. SERUM. Serum samples (100 µL) were deproteinized by an addition of 300 µL of cold acetonitrile (1:3 v/v) and homogenized for 15 min in a vortex. After a 10 min ice bath, samples were centrifuged (16000 x g, 4°C, 20 min) and stored at -20°C until the start of sample treatment. The resulting supernatants were filtered through 0.22 µm for LC-MS, 80 µL were transferred into an analytical vial for their analysis. For CE-MS, 80 µL of the supernatants were evaporated to dryness using a Speedvac Concentrator and reconstituted in 80 µL of MilliQ® water containing an internal standard (0.2 mM L-methionine sulfone) and 0.1 M FA. Finally, for GC-MS, 120 µL of the corresponding aliquots were evaporated to dryness using a Speedvac Concentrator, followed by the addition of 10 µL of O-methoxyamine hydrochloride (15 mg/mL) in pyridine for methoximation. After gently vortexing, the vials were incubated in darkness at room temperature for 16 h. Then, 10 µL of BSTFA with 1 % TMCS (v/v) were added and samples were vortexed for 5 min, silylation was carried out for 1 h at 70 °C and finally 120 µL of C18:0 methyl ester (10 mg/L in heptane) were added as an internal standard and samples were mixed again by gentle vortexing. Eight blank samples were prepared for GC-MS by the same procedure of extraction and derivatization. |
| Sampleprep Protocol Filename: | Protocolsamples.docx |
| Processing Method: | Lyophilization, homogenization, protein precipitation and metabolite extraction |
| Processing Storage Conditions: | On ice |
