Summary of Study ST002578

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001663. The data can be accessed directly via it's Project DOI: 10.21228/M8R72W This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002578
Study TitleSteady State Metabolomics Study in SMARCA4 mutant(BIN-67) cells cultured in the absence of glucose or glutamine
Study Summarysteady-state abundances of all TCA cycle intermediates were more severely reduced in BIN-67 cells deprived of glutamine than those deprived of glucose
Institute
McGill University
DepartmentBiochemistry
LaboratorySidong Huang Lab
Last NameFu
First NameZheng
AddressMcIntyre Medical Sciences Building, 3655 promenade Sir-William-Osler
Emailzheng.fu2@mail.mcgill.ca
Phone5143985446
Submit Date2023-04-24
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailGC-MS
Release Date2023-05-22
Release Version1
Zheng Fu Zheng Fu
https://dx.doi.org/10.21228/M8R72W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002686
Sampleprep Summary:Cells were washed twice in cold saline solution (NaCl, 0.9 g/l) and metabolites were extracted with 1 ml 80% ice-cold methanol (GC/MS grade). After 2 rounds 10 min sets of sonication (30 seconds on/30 seconds off at high intensity) on slurry ice using a Bioruptor UCD-200 sonicator, the homogenates were centrifuged at 14,000 × g at 4 °C for 10 min. Supernatants were collected and supplemented with internal control (800 ng myristic acid-D27) and dried in a cold vacuum centrifuge (Labconco) overnight. The dried pellets were reconstituted with 30 μL of 10 mg/mL methoxyamine-HCl in pyridine, and incubated for 30 min at room temperature. Samples were then derivatized with MTBSTFA for 30 min at 70 °C. A volume of 1 μL of sample was injected splitless with an inlet temperature of 280 oC into the GC (7890, Agilent)/MS (5975C, Agilent) instrument.
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