Summary of Study ST002721

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001687. The data can be accessed directly via it's Project DOI: 10.21228/M8NB07 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002721
Study TitleRepeated Exposure to Wood Smoke Alters Pulmonary Gene and Metabolic Profiles
Study SummaryThis project aims to evaluate the association of eucalyptus wood smoke exposure with perturbations to lung metabolism in a pre-clinical rat model. This cross-sectional study of male Long-Evans rats were exposed whole body to eucalyptus smoke for 4 consecutive days on week 1, followed by a 3-day break, and 3 subsequent days of smoke exposure on week 2. The smoke exposures were set to a target CO of 10ppm for the low smoke group and 20ppm for the high smoke group. Animal were sacrificed and lungs were extracted for metabolomics analysis. Exposures to eucalyptus smoke and biological perturbations to lung metabolism will be associated with known metabolic dysregulation associated with smoke exposures.
Institute
Emory University
DepartmentSchool of Medicine
LaboratoryClinical Biomarkers Laboratory
Last NameTran
First NameViLinh
Address615 Michael St, Suite 225, Atlanta, GA 30322, USA
Emailvtran6@emory.edu
Phone4047275091
Submit Date2023-05-12
Num Groups3
Total Subjects8
Num Males8
Study CommentsClinical Biomarker Laboratory pooled plasma samples were used
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-05-13
Release Version1
ViLinh Tran ViLinh Tran
https://dx.doi.org/10.21228/M8NB07
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002833
Sampleprep Summary:Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, lung samples were removed from storage at -80 degrees C and thawed on ice. The 20-30mg of each lung tissue samples was then mixed with ice cold LC-MS grade acetonitrile: water mixture (Sigma-Aldrich; 2:1 ration; 15uL per mg tissue) which contains 2.5uL per 100uL of internal standard solution with eight stable isotopic chemicals selected to cover a range of chemical properties. Samples are then sonicated on ice to ensure metabolite extraction as previously described (Chandler et al (2016) AJ Physiology; Hu et al. (2019) AJ Pathology). Following sonication, tissue lysates are then equilibrated for 30 mins on ice, upon which precipitated proteins are removed by centrifuge (16.1xg at 4C for 10 mins). The resulting supernatant containing metabolite extract (100uL) is removed, added to a low volume autosampler vial and maintained at 4C until analysis (<22h).
Sampleprep Protocol Filename:EmoryUniversity_HRM_SamplePreparation_tissue_062016_v1.pdf
Processing Storage Conditions:-80℃
Extract Storage:-80℃
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