Summary of Study ST003253

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002019. The data can be accessed directly via it's Project DOI: 10.21228/M8N23M This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003253
Study Title	Metabolomics studies on mouse liver samples on a Western diet
Study Summary	Targeted metabolomics was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).to measure polar metabolites in both positive and negative ionization mode on cardiac mice tissue acquired after a 30 week dietary intervention.
Institute	University of Sydney
Department	School of Medical Sciences
Laboratory	Cardiometabolic Disease
Last Name	Liu
First Name	Renping
Address	Charles Perkins Centre
Email	renping.liu@sydney.edu.au
Phone	+61432953638
Submit Date	2024-05-03
Num Groups	4
Total Subjects	35
Num Males	35
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	API-MS
Release Date	2024-08-26
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003379
Sampleprep Summary:	Approximately 50 mg of ground liver tissue was weighed into 2 mL Eppendorf tube and subjected to a two-phase extraction protocol involving tissue, ice-cold extraction medium (methanol:water, and chloroform). Samples were then centrifuged at 14 000 x g at 4 °C for 25 min. The aqueous layer was transferred into a new microfuge tube and reconstituted in the acetonitrile/methanol/formic acid (75:25:0.2; v/v/v, HPLC grade; Thermo Fisher Scientific) for the HILIC analysis, and acetonitrile/methanol (25:25; v/v/v, HPLC grade) for the AMIDE and NAD analysis.

Sampleprep ID:

SP003379

Sampleprep Summary:

Approximately 50 mg of ground liver tissue was weighed into 2 mL Eppendorf tube and subjected to a two-phase extraction protocol involving tissue, ice-cold extraction medium (methanol:water, and chloroform). Samples were then centrifuged at 14 000 x g at 4 °C for 25 min. The aqueous layer was transferred into a new microfuge tube and reconstituted in the acetonitrile/methanol/formic acid (75:25:0.2; v/v/v, HPLC grade; Thermo Fisher Scientific) for the HILIC analysis, and acetonitrile/methanol (25:25; v/v/v, HPLC grade) for the AMIDE and NAD analysis.