Summary of Study ST003297
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002048. The data can be accessed directly via it's Project DOI: 10.21228/M8WG0S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003297 |
| Study Title | Metabolomic profiling of cultured TRAMP-C2 cells in the presence or absence of PD-L1. |
| Study Summary | Recent evidence suggests that PD-L1, well-known as the ligand of the immune inhibitory receptor PD-1, can have cell-intrinsic effects in cancer and immune cells. One such cell-intrinsic effect is modulation of cellular metabolism, including regulation of mTOR activity and glycolysis. Here, we analyzed the metabolome of cultured mouse prostate cancer cells (TRAMP-C2) expressing PD-L1 or with PD-L1 deleted via CRISPR/Cas9. |
| Institute | University of Ottawa |
| Last Name | Hodgins |
| First Name | Jonathan |
| Address | 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada |
| jonathanhodgins17@gmail.com | |
| Phone | 613-562-5800 |
| Submit Date | 2024-05-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-28 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003425 |
| Sampleprep Summary: | Samples were mixed with 230 uL of 1:1 methanol:water, along with 6 washed 1.4 mm ceramic beads. Samples were vortexed for 10 s and cell lysis was done by beating for 60 s at 2000 rpm (bead beating was done twice) after adding 220 µL of acetonitrile. Samples were then incubated with a 2:1 dichloromethane:water solution on ice for 10 minutes. The polar and non-polar phases were separated by centrifugation at 4000g for 10 minutes at 1°C. The upper polar phase was dried using a refrigerated CentriVap Vacuum Concentrator at -4°C (LabConco Corporation, Kansas City, MO). Samples were resuspended in water. |
| Processing Storage Conditions: | On ice |
