Summary of Study ST003340
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002071. The data can be accessed directly via it's Project DOI: 10.21228/M8X835 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003340 |
Study Title | Effect of feeding and the mTORC1 activity on metabolism in Caenorhabditis elegans |
Study Summary | Cellular growth and organismal development are remarkably complex processes that require the nutrient-responsive kinase mTORC1. Anticipating that important mTORC1 functions remained to be identified, we employed genetic and bioinformatic screening in C. elegans to uncover mechanisms of mTORC1 action. Here we show that during larval growth nutrients induce an extensive reprogramming of gene expression and alternative mRNA splicing by acting through mTORC1. mTORC1 regulates mRNA splicing and production of protein-coding mRNA isoforms largely independently of its target p70S6K, by increasing the activity of the serine/arginine-rich (SR) protein RSP-6 (SRSF3/7) and other splicing factors. mTORC1-mediated mRNA splicing regulation is critical for growth, mediates nutrient control of mechanisms that include energy, nucleotide, amino acid, and other metabolic pathways, and may be conserved in humans. Although mTORC1 inhibition delays aging, mTORC1-induced mRNA splicing promotes longevity, suggesting that when mTORC1 is inhibited enhancement of this splicing might provide additional anti-aging benefits. |
Institute | Hiroshima University |
Last Name | Ogawa |
First Name | Takafumi |
Address | 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima, 739-8530, Japan |
takafumi-ogawa@hiroshima-u.ac.jp | |
Phone | +81-80-9790-5278 |
Submit Date | 2024-07-17 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-11-20 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003468 |
Sampleprep Summary: | The samples were then centrifuged at 16,000g for 15 minutes at 4ºC, and the supernatant was passed through an Ultrafree-MC VV Centrifugal Filter, 0.1 µm (Millipore) by centrifuging at 9100g. The filtrate was concentrated and dried using a Speedvac (Thermo Scientific), and resuspended in 50:50 acetonitrile:water for LC-MS analysis. Metabolites were resolved on a Vanquish U-HPLC system coupled to a Q Exactive HF-X hybrid quadrupole-orbitrap mass spectrometer (ThermoFisher) with a HESI source operating in negative ion mode. |