Summary of Study ST003425

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002117. The data can be accessed directly via it's Project DOI: 10.21228/M8ZV6G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003425
Study Title	Targeted free fatty acids metabolomics studies on serum, cecal content, cecum tissue, chow diet, and Tritrichomonas mu cells
Study Summary	Serum, cecal content, cecum tissue samples of murine, chow diet, and T.mu cells were collected to perform the targeted free fatty acids metabolome analysis. The aim of this study was to verify that whether T.mu could release free PUFA (especially ARA) to the intestinal tract of its host by comparing the PUFA concentration in the freshly isolated T.mu cells, chow diet, cecal content and serum of the host (Mus musculus).Based on the result of this targeted free fatty acids metabolomics studies, we found that T.mu could release ARA to the intestinal tract of its host and increase the concentration of ARA in its host's intestinal tract.
Institute	Xuzhou medical university
Last Name	Kou
First Name	Yanbo
Address	Tongshan road 209, Xuzhou, Jiangsu, 221004, China
Email	fightingkyb@163.com
Phone	+86-051683262123
Submit Date	2024-08-20
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2024-09-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003559
Sampleprep Summary:	The weighed cecal content, cecum tissue, serum, animal chow diet powder, or enumerated T.mu cells were transferred into new 2 mL EP tubes followed extraction with 500 μL extracting solution [Isopropanol : n-Hexane = 2:3 (V:V)], including 0.2 mg/L internal standard). After homogenized in a ball mill for 4 min at 40 Hz and a 5 min ultrasound treatment, the samples were centrifuged 16200 × g for 15 min at 4 °C. Then the supernatants were transferred into new 2 mL EP tubes followed with nitrogen blow dry. The dried samples were resuspended in 500 μL of methanol: trimethylsilyl diazomethane solution (1:2), after standing 30 min at room temperature, another nitrogen blow dry was performed. Then, the samples were resuspended in 160 μL of n-hexane followed with a centrifugation of 16200 × g for 1 min. Finally, the supernatants were transferred into new vials for GC-MS analysis.

Sampleprep ID:

SP003559

Sampleprep Summary:

The weighed cecal content, cecum tissue, serum, animal chow diet powder, or enumerated T.mu cells were transferred into new 2 mL EP tubes followed extraction with 500 μL extracting solution [Isopropanol : n-Hexane = 2:3 (V:V)], including 0.2 mg/L internal standard). After homogenized in a ball mill for 4 min at 40 Hz and a 5 min ultrasound treatment, the samples were centrifuged 16200 × g for 15 min at 4 °C. Then the supernatants were transferred into new 2 mL EP tubes followed with nitrogen blow dry. The dried samples were resuspended in 500 μL of methanol: trimethylsilyl diazomethane solution (1:2), after standing 30 min at room temperature, another nitrogen blow dry was performed. Then, the samples were resuspended in 160 μL of n-hexane followed with a centrifugation of 16200 × g for 1 min. Finally, the supernatants were transferred into new vials for GC-MS analysis.