Summary of Study ST003492
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002141. The data can be accessed directly via it's Project DOI: 10.21228/M8VZ4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST003492 |
| Study Title | Metabolomic profiling of the secretome in normal primary human pulmonary artery smooth muscle cells |
| Study Summary | Growth medium of human pulmonary artery smooth muscle cells (PASMCs) was conditioned for 24 hours and analyzed by LC-MS, termed conditioned medium (CM). Comparing to fresh complete vascular smooth muscle cell growth medium (GM), LC-MS profiling screened 138 metabolites and identified that three branched chain alpha-ketoacids, namely KIC, KIV, and KMV are the most abundant metabolites in CM of human PASMCs. |
| Institute | Peking University |
| Department | Department of Toxicology |
| Last Name | Xiao |
| First Name | Wusheng |
| Address | 38 Xueyuan Rd, Haidian District, Beijing, Beijing, 100191, China |
| wxiao@bjmu.edu.cn | |
| Phone | +1-010-82801628 |
| Submit Date | 2023-08-07 |
| Analysis Type Detail | MALDI |
| Release Date | 2024-10-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003627 |
| Sampleprep Summary: | Metabolites in equal volume of GM and CM were extracted using 100% -80 degree prechilled LC-MS grade methanol. The final concentration of methanol was 80%. Proteins were removed by centrifugation and supernatants were collected and evaporate on an Integrated SpeedVac System to the starting volume (~200 uL). Twenty microliters of the resulting medium samples were loaded into a LC-MS sampling tube for analysis. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
