Summary of Study ST003531
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002173. The data can be accessed directly via it's Project DOI: 10.21228/M8R23J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003531 |
| Study Title | Micropeptide hSPAR, a glutamine regulator, suppresses tumor growth via TRIM21-P27KIP1-mTOR pathway - human MDA-MB-231 breast cancer cell |
| Study Summary | hSPAR, a microprotein, is capable of specifically inhibiting the mTOR signaling activity and cell proliferation in MDA-MB-231 cells. Glutamine, an essential amino acid, plays a crucial role in regulating the mTOR signal. Our data indicate that in MDA-MB-231 cells, overexpressing hSPAR inhibits glutamine uptake, consequently suppressing the activation of the mTOR signaling. |
| Institute | University of Science and Technology of China |
| Last Name | Wang |
| First Name | Wei |
| Address | Division of Life Sciences and Medicine, 443 Huangshan Road, Hefei city, Anhui Province |
| WW571@mail.ustc.edu.cn | |
| Phone | 18604523231 |
| Submit Date | 2024-10-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-06 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003667 |
| Sampleprep Summary: | The sample was thawed on ice, 100 μL of ultrapure water extract (containing protease inhibitors, PMSF and EDTA) was added to resuspend the cell pellet. Divide 50 μL cell suspension and add 200 µL of methanol (precooled at -20°C) and vortexed for 2 min under the condition of 2500 rpm. The sample was frozen in liquid nitrogen for 5 min, removed on ice for 5 min, after that, the sample was vortexed for 2 min.The previous step was repeated for 3 times. The sample was centrifuged at 12000 rpm for 10 min at 4°C. Take 200 μL of supernatant into a new centrifuge tube and place the supernatant in -20°C refrigerator for 30 min. Then the supernatant was centrifuged at 12000 rpm for 10 min at 4°C. After centrifugation, transfer 180 μL of supernatant through Protein Precipitation Plate for further LC-MS analysis. The remaining 50 μL cell suspension was frozen and thawed for 3 times, centrifuged at 12,000 rpm for 10 min, and the supernatant was taken to determine the protein concentration by BCA Protein Assay kit. |
| Sampleprep Protocol Filename: | USTC_Protocol.pdf |
