Summary of Study ST003643
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002253. The data can be accessed directly via it's Project DOI: 10.21228/M8DC25 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003643 |
| Study Title | Metabolite analysis for WT and BnaMYB52 mutants by LC-MS/MS |
| Study Summary | BnaA09.MYB52 directly targets the BnaBAN promoters and promotes BnaBAN expression in Brassica napus. BAN, encoding anthocyanidin reductase that converts anthocyanidins to 2,3- cis-flavan-3-ols compounds (proanthocyanidins starter units), is involved in the flavonoid biosynthesis pathway. Thus, Metabolite analysis was conducted to detect the content of flavonoid in WT (Wild-type), OE (BnaA09.MYB52 overexpression lines in the genetic background Westar) and mutants (four homologous genes of BnaMYB52 knocked out) plants. About 0.1 g mature seeds were collected from WT, OE and mutant plants. Metabolites analysis demonstrated that BnaMYB52 positively regulated the content of several metabolites (such as L-phenylalanine, p-coumaric acid, grosvenorine and astragalin) in flavonoid pathway. |
| Institute | Huazhong Agricultural University |
| Last Name | Jiang |
| First Name | Ye |
| Address | Shi Zishan Street 1th, Wuhan, Hubei, 430070, China |
| vyejiang@163.com | |
| Phone | 13697353446 |
| Submit Date | 2024-12-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-26 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003780 |
| Sampleprep Summary: | Mature seeds were ground into powder in liquid nitrogen, and 0.1 g of dry powder was weighed. The metabolites were extracted overnight under 4℃ with 1.0 mL 70% methanol (V/V) containing 0.1 mg/l acyclovir (an internal standard). After centrifugation at 10,000 g for 5 min, the supernatant was kept and filtered with a membrane (0.22 μm pore size). |
| Processing Method: | Grind in extraction solution |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | Extracted overnight under 4℃ with 1.0 mL 70% methanol (V/V) containing 0.1 mg/l acyclovir (an internal standard). After centrifugation at 10,000 g for 5 min, the supernatant was kept and filtered with a membrane |
| Extract Storage: | -80℃ |
