Summary of Study ST003790
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002366. The data can be accessed directly via it's Project DOI: 10.21228/M8SZ6Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003790 |
| Study Title | Fecal metabolomics of B16-OVA tumor-bearing mice fed chow or low and high fiber purified diets and treated with isotype control or anti-PD-1 antibody |
| Study Summary | Immune checkpoint blockade (ICB) has transformed cancer treatment, but success rates remain limited. Recent research suggests dietary fiber enhances ICB response in mice and patients through microbiome-dependent mechanisms. Yet, the robustness of this effect across cancer types and dietary contexts remains unclear. Specifically, prior literature compared grain-based chow (high fiber) to low-fiber purified diet, but these diets differ also on other dimensions including phytochemicals. Here we investigated, in mice fed grain-based chow or purified diets with differing quantities of isolated fibers (cellulose and inulin), the gut microbiome, metabolite levels and ICB activity in multiple tumor models. Isolated fibers shifted multiple microbial taxa toward chow-like levels, although diet-type (chow vs. purified) had a stronger impact on gut-microbiome composition. Metabolomic profiles were relatively similar between mice fed high- and low-fiber purified diets, but differed massively between mice fed purified diets or chow, identifying the factor as diet type, independent of fiber. Tumor growth studies in multiple murine models revealed that fiber has a weaker impact on ICB (anti-PD-1) efficacy than previously reported. While diet impacted ICB activity in some models, the effect was not directionally consistent. In no model did we observe the pattern expected if fiber controlled ICB efficacy: strong efficacy in both chow and high-fiber purified diet but low efficacy in low-fiber purified diet. Thus, dietary fiber appears to have limited or inconsistent effect on ICB efficacy in mouse models, and other dietary factors that correlate with fiber intake may underlie the clinical correlations between fiber consumption and immunotherapy outcomes. |
| Institute | Princeton University |
| Last Name | Roichman |
| First Name | Asael |
| Address | Lewis-Sigler Institute for Integrative Genomics Princeton University Carl Icahn Laboratory, Washington Road Princeton, NJ 08544 |
| asael100@gmail.com | |
| Phone | 9084101710 |
| Submit Date | 2025-03-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-03-31 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003930 |
| Sampleprep Summary: | Frozen feces samples were transferred to Eppendorf tubes with ceramic beads on dry ice and disrupted by cryomill (Retsch). About 10 mg of homogenized powder was weighed and extracted by ice-cold acetonitrile: methanol: water (40:40:20, supplemented with 0.5 % vol formic acid). Extracts were vortexed for 10 s, kept on dry ice for 10 min, and neutralized by NH4HCO3 (15% in water, 8.8% vol/vol of extraction buffer was used). Neutralized extracts were vortexed for 10 s again, kept on dry ice for 1h, and centrifuged at 21, 380 g, 4°C for 20 min. Supernatants were collected for LC-MS analysis. |
