Summary of Study ST003808

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002382. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ7Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003808
Study Title	Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division
Study Summary	Peroxisomes play a crucial role in cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the gatekeeper enzyme of the pentose phosphate pathway, is primarily localized in the cytosol. However, studies have reported its presence in peroxisomes as well. This project aims to determine the function of G6PD on the peroxisomal membrane. In this study, we overexpressed G6PD in either the cytosol or on the peroxisomal membrane of mammary epithelial stem-like cells (hMECs). By comparing their lipidomic profiles, we found that peroxisomal membrane-associated G6PD provides NADPH, which feeds into peroxisomal ether lipid synthesis.
Institute	University of Helsinki
Department	Faculty of Biological and Environmental Sciences
Laboratory	Katajisto Laboratory
Last Name	Hien
First Name	Bui
Address	Biocenter 1, Viikinkaari 9
Email	hien.bui@helsinki.fi
Phone	+358294159407
Submit Date	2025-03-06
Raw Data Available	Yes
Raw Data File Type(s)	cdf, mzdata.xml
Analysis Type Detail	GC-MS/LC-MS
Release Date	2025-03-24
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003948
Sampleprep Summary:	GC-MS: Fatty acyl (FA) and alkenyl chains, and fatty alcohols (FOHs) were analysed by gas chromatography (GC). Aliquots of cell pellet Folch-extracts were evaporated near to dryness under nitrogen flow and the extracted lipids were transmethylated by heating with 1% H2SO4 in methanol under nitrogen atmosphere. This converts FAs to their methyl esters (FAMEs), phospholipid-derived alkenyl chains to dimethyl acetals (DMAs) and leaves FOHs (1-ol) intact. After adding water, the FAMEs, DMAs and FOHs were extracted with hexane, and the sample solution was dried, and concentrated. LC-MS: Lipids were extracted from cell pellets (at least from one million cells) according to Folch et al (PMID: 13428781), and dissolved in chloroform/methanol 1:2 (v/v). Internal standards [EquiSPLASH® internal standard mixture and Ceramide (Cer) 18:1;O2/17:0, both from Merck] were added and samples were analyzed with LC-MS/MS.

Sampleprep ID:

SP003948

Sampleprep Summary:

GC-MS: Fatty acyl (FA) and alkenyl chains, and fatty alcohols (FOHs) were analysed by gas chromatography (GC). Aliquots of cell pellet Folch-extracts were evaporated near to dryness under nitrogen flow and the extracted lipids were transmethylated by heating with 1% H2SO4 in methanol under nitrogen atmosphere. This converts FAs to their methyl esters (FAMEs), phospholipid-derived alkenyl chains to dimethyl acetals (DMAs) and leaves FOHs (1-ol) intact. After adding water, the FAMEs, DMAs and FOHs were extracted with hexane, and the sample solution was dried, and concentrated. LC-MS: Lipids were extracted from cell pellets (at least from one million cells) according to Folch et al (PMID: 13428781), and dissolved in chloroform/methanol 1:2 (v/v). Internal standards [EquiSPLASH® internal standard mixture and Ceramide (Cer) 18:1;O2/17:0, both from Merck] were added and samples were analyzed with LC-MS/MS.