Summary of Study ST001148
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000767. The data can be accessed directly via it's Project DOI: 10.21228/M8J68Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001148 |
Study Title | Effect of cell harvesting technique and storage on metabolic profiles in human skin fibroblasts |
Study Type | Method |
Study Summary | Human skin fibroblasts were cultured in MEM media supplemented with 15% FBS. Cells were harvested using (i) trypsinization, (ii) scraping, (iii) methanol fixation and scraping, (iV) methanol fixation, scraping, and drying. Targeted metabolomics analysis of organic acids, amino acids, and acycarnitines was conducted at Mayo Clinic Metabolomics Core Facility. |
Institute | Mayo Clinic |
Department | Neurology |
Last Name | Wilkins |
First Name | Jordan |
Address | 200 First St SW |
wilkins.jordan@mayo.edu | |
Phone | 5072933857 |
Submit Date | 2019-02-27 |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
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Subject:
Subject ID: | SU001213 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 21 |
Gender: | Male |
Cell Biosource Or Supplier: | Mayo Clinic |
Cell Strain Details: | 10268 |
Cell Passage Number: | 10 |
Species Group: | Mammals |