Summary of Study ST001478

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001002. The data can be accessed directly via it's Project DOI: 10.21228/M85T2G This work is supported by NIH grant, U2C- DK119886.

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Study IDST001478
Study TitleNMR spectroscopy analysis reveals altered metabolic homeostasis in Arabidopsis seedlings treated with a cytokinesis inhibitor
Study SummaryIn plant cytokinesis, de novo formation of a cell plate that evolves into the new cell wall, partitions the cytoplasm of the dividing cell. Cell plate formation involves highly orchestrated vesicle accumulation, fusion, and membrane network maturation supported by the temporary integration of elastic and pliable callose. The small molecule, Endosidin 7 (ES7), arrests late cytokinesis in Arabidopsis by inhibiting callose deposition at the cell plate. Its effect is specific, as it does not broadly affect endomembrane trafficking or cytoskeletal organization. It has emerged as a very valuable tool for dissecting this essential plant process. In order to gain deeper insights regarding its mode of action and the effects of cytokinesis inhibition on overall plant growth, we investigated the effect of ES7 through a nuclear magnetic resonance (NMR) spectroscopy metabolomics approach. In this case study, profiles of Arabidopsis leaf and root tissues were analyzed at different growth stages and ES7 exposure levels. The results show tissue-specific changes in the plant metabolic profile across a developmental gradient, and the effect that ES7 treatment has on the corresponding metabolome. The ES7 induced profile suggests metabolic compensations in central metabolism pathways in response to cytokinesis inhibition. Further, this study shows that long-term treatment of ES7 disrupts the homeostasis of primary metabolism in Arabidopsis seedlings, likely via alteration of hormonal regulation.
Institute
California State University Fresno
Last NameKrishnan
First NameKrish
AddressDepartment of Chemistry
Emailkrish@csufresno.edu
Phone559-278-7944
Submit Date2020-06-25
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2021-06-28
Release Version1
Krish Krishnan Krish Krishnan
https://dx.doi.org/10.21228/M85T2G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU001552
Subject Type:Plant
Subject Species:Arabidopsis thaliana
Taxonomy ID:3702
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