Summary of Study ST001983

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001259. The data can be accessed directly via it's Project DOI: 10.21228/M8ZX2N This work is supported by NIH grant, U2C- DK119886.

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Study IDST001983
Study TitleMetabolomic Fingerprinting of Human High Grade Serous Ovarian Carcinoma Cell Lines
Study SummaryFocusing on defining the metabolomic basis of intratumoral heterogeneity in ovarian cancer, the metabolic diversity of a panel of high grade serous ovarian carcinoma (HGSOC) cell-lines we investigated using a metabolomics platform that interrogate 731 compounds. Metabolic fingerprinting followed by 2-dimensional and 3-dimensional principal component analysis defined the heterogeneity of the HGSOC cells and clustered them into five distinct metabolic groups. An overall increase in the metabolites associated with aerobic glycolysis and phospholipid metabolism were observed in the majority of the cancer cells. A preponderant increase in the levels of metabolites involved in trans-sulfuration and glutathione synthesis was also observed. Subsets of HGSOC cells showed an increase in the levels of 5-Hydroxytryptamine, gamma-aminobutyric acid, or glutamate, pointing to their potential role as oncometabolites. In summary, our results identify increased glycolysis, phospholipid metabolism and amino acid metabolism with the resultant increase in the levels of 5-Hydoxytryptamine, GABA, and Glutamate as metabolomic correlates underlying the heterogeneity of ovarian cancer cell lines.
Institute
University of Oklahoma Health Sciences Center
DepartmentCell Biology
LaboratoryDanny N Dhanasekaran
Last NameJayaraman
First NameMuralidharan
Address975 NE 10th street, BRC1470, Oklahoma City, Oklahoma, 73104, USA
EmailMuralidharan-Jayaraman@ouhsc.edu
Phone4052718001 x 30492
Submit Date2021-09-20
Num Groups2
Total Subjects30
Num Females15
Study CommentsOvarian cancer cell lines
Analysis Type DetailLC-MS
Release Date2021-12-01
Release Version1
Muralidharan Jayaraman Muralidharan Jayaraman
https://dx.doi.org/10.21228/M8ZX2N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU002064
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female
Cell Biosource Or Supplier:OVCAR4 cell line was obtained from Dr. Thomas Hamilton (Fox Chase Cancer Center, PA) and OVCAR8 cells were from National Cancer Institute (NCI). Kuramochi, TYKNU, OVKATE and OVSAHO cell lines were from the JCRB Cell Bank, Tokyo, Japan. SNU119, SNU251, ES-2, OVCAR3, CAOV3 and OV90 cells were from Seoul National University, Seoul, Korea, and COV362, OAW28 and COV318 cells were purchased from Sigma-Aldrich (St. Louis, MO).
Cell Passage Number:3 and 4
Cell Counts:20 million cells
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