Summary of Study ST002140

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001355. The data can be accessed directly via it's Project DOI: 10.21228/M8K70K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002140
Study TitleMitochondrial respiration in B lymphocytes is essential for humoral immunity by controlling flux of the TCA cycle
Study SummaryThe function of mitochondrial respiration during B cell fate decisions and differentiation 55 remained equivocal. This study reveals that selection for mitochondrial fitness occurs during B 56 cell activation and is essential for subsequent plasma cell differentiation. By expressing a 57 mutated mitochondrial helicase in transitional B cells, we depleted mitochondrial DNA during 58 B cell maturation, resulting in reduced oxidative phosphorylation. Although no changes in 59 follicular B cell development were evident, germinal centers, class switch recombination to 60 IgG, plasma cell maturation and humoral immunity were diminished. Defective oxidative 61 phosphorylation led to aberrant flux of the tricarboxylic acid cycle and lowered the amount of 62 saturated phosphatidic acid. Consequently, mTOR activity and BLIMP1 induction were 63 curtailed whereas HIF1 _and glycolysis were amplified. Exogenous phosphatidic acid 64 increased mTOR activity in activated B cells. Hence, mitochondrial function is required and 65 selected for in activated B cells for the successful generation of functional plasma cells.
Institute
University of Erlangen-Nürnberg
DepartmentDivision of Molecular Immunology.Universitätsklinikum Erlangen, Nikolaus Fibinger Zentrum
LaboratoryProf. Mielenz
Last NameMielenz
First NameDirk
AddressNikolaus-Fiebiger-Zentrum, Glückstraße 6, 91054 Erlangen
Emaildirk.mielenz@fau.de
Phone++49 9131 8539105
Submit Date2022-04-06
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS/MS(Dir. Inf.)
Release Date2022-05-02
Release Version1
Dirk Mielenz Dirk Mielenz
https://dx.doi.org/10.21228/M8K70K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Subject:

Subject ID:SU002225
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male and female
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