Summary of Study ST000055
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000052. The data can be accessed directly via it's Project DOI: 10.21228/M8H01X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000055 |
| Study Title | Effect of kinase inhibitors on FLT3-ITD AML cell metabolomes |
| Study Summary | FLT3-ITD AML cells (MR2) obtained from mice were treated with two MEK kinase inhibitors (GSK and AZD) at 10 uM versus media only control. Conditioned media aliquots and cellular fractions comprised of two aliquots (tecnical replicates) for LC-MS metabolomic and one for Western blot analyses were collected at 0, 4, 24, and 48 hours. The experiment was repeated three times. HPLC-MS data were acquired for 24 samples from one biological experiment. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-06-06 |
| Num Groups | 12 |
| Total Subjects | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Uploaded File Size | 1.2 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-06-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000075 |
| Treatment Summary: | MEK kinase inhibitors |
| Treatment Compound: | GSK | AZD |
| Treatment Route: | added to media |
| Treatment Dose: | 10nM | 10nM |
| Treatment Vehicle: | dilutions made in 1X sterile PBS (orginally dissolved in DMSO) |
| Cell Storage: | liquid nitrogen, live maintenance in 5% CO2, humidified incubator |
| Cell Growth Container: | T-75 culture flask |
| Cell Growth Config: | suspension |
| Cell Growth Rate: | double ~every 20 hours |
| Cell Media: | 50:50 DMEM:IMDM + 10% FBS, 1% P/S |
| Cell Harvesting: | Transfer 1 mL to new flask containing 10 mL of fresh culture media |
| Cell Pct Confluence: | maintained at ~ 2 x10^6/mL |
