Summary of Study ST000113
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000102. The data can be accessed directly via it's Project DOI: 10.21228/M88C7T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000113 |
| Study Title | SIRM Analysis of human P493 cells under hypoxia in [U-13C/15N] labeled Glutamine medium (Positive ion mode FTMS) |
| Study Type | tracer |
| Study Summary | SIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in [U-13C/15N] labeled Glutamine medium |
| Institute | University of Kentucky |
| Department | Toxicology |
| Last Name | Fan |
| First Name | Teresa |
| twmfan@gmail.com | |
| Submit Date | 2014-10-08 |
| Num Groups | 4 |
| Total Subjects | 8 |
| Study Comments | For protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., Bose, S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. L.J., Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., and Dang, C.V. Cell Metabolism 15, 110 (2012). 13C/15N Gln was used as the tracer in this study, even though the analysis did not explicity look for 15N isotopologues. |
| Raw Data Available | No |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2015-02-03 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000134 |
| Treatment Protocol Comments: | tet-normox-13C/15N-Gln: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln | normox-13C/15N-Gln: cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln | hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with [U-13C/15N]-Gln | tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln |
