Summary of Study ST000133

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000118. The data can be accessed directly via it's Project DOI: 10.21228/M8B594 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000133
Study Title	1H NMR Metabolomics Study of Metastatic Melanoma in C57BL/6J Mouse Spleen
Study Type	Tissue Extracts Comparison
Study Summary	Tissue extracts from metastatic melanoma mouse spleen and controls were compared via NMR based metabolomic analysis
Institute	Pacific Northwest National Laboratory
Department	Fundamental & Computational Sciences
Last Name	Hu
First Name	Jianzhi
Email	jianzhi.hu@pnnl.gov
Submit Date	2015-01-08
Num Groups	1
Total Subjects	12
Raw Data Available	Yes
Uploaded File Size	140 K
Analysis Type Detail	NMR
Release Date	2015-01-08
Release Version	1

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Treatment:

Treatment ID:	TR000154
Treatment Summary:	A total of 12 six-week-old C57BL/6J male mice at the age of week-7 the mice were randomly divided into two groups, an experimental group (n=7) that received subcutaneous injection with suspended 105 B16-F10 tumor cells at four flank locations on each leg, and a control group (n=5) that were injected with the same amount of PBS at same places. The animals were fed a standard diet and housed one mouse per cage and maintained in a temperature-controlled room (22-25°C, 45% humidity) on a 12:12h dark-light cycle. All mice, each of which was weighed weekly, were allowed free access to water and food before being sacrificed by CO2 asphyxiation at the age of fourteen weeks.

Treatment ID:

TR000154

Treatment Summary:

A total of 12 six-week-old C57BL/6J male mice at the age of week-7 the mice were randomly divided into two groups, an experimental group (n=7) that received subcutaneous injection with suspended 105 B16-F10 tumor cells at four flank locations on each leg, and a control group (n=5) that were injected with the same amount of PBS at same places. The animals were fed a standard diet and housed one mouse per cage and maintained in a temperature-controlled room (22-25°C, 45% humidity) on a 12:12h dark-light cycle. All mice, each of which was weighed weekly, were allowed free access to water and food before being sacrificed by CO2 asphyxiation at the age of fourteen weeks.