Summary of Study ST000134
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000119. The data can be accessed directly via it's Project DOI: 10.21228/M86C76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000134 |
| Study Title | Monitoring In Vitro Response of Selenium-Treated Prostate Cells by 1H NMR Spectroscopy |
| Study Type | Metabolite level response after treatment with organoselenium |
| Study Summary | Metabolomics analysis was performed on DU145 prostate cancer cells and PNT1A non-tumorigenic prostate cells after treatment with selenomethionine and Se-methylselenocysteine using 800 MHz Bruker NMR spectrometer on 18 cell samples. |
| Institute | Purdue University |
| Department | Biology/Chemistry |
| Laboratory | Biology/Chemistry |
| Last Name | Isaac-Lam |
| First Name | Meden |
| Address | 1401 S US Hwy 421, Westville, IN 46391 |
| isaaclam@pnc.edu | |
| Phone | 219-785-5776 |
| Submit Date | 2015-01-13 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | No |
| Analysis Type Detail | NMR |
| Release Date | 2015-01-13 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000155 |
| Treatment Summary: | Cells were grown to 80% confluence in Petri dishes (50 mm in diameter) in 4.5% CO2 at 37 OC for 5-6 days for full attachment to the substratum. After aspirating the old growth medium, 3 mL of SeM or SeMSC (300 mM) in fresh pre-warmed medium at 37 ºC was added to each dish. |
| Treatment Compound: | Selenomethionine / Se-methylselenocysteine |
| Treatment Dose: | 300 mM |
| Treatment Dosevolume: | 3 mL |
| Treatment Doseduration: | 24 hrs |
| Treatment Vehicle: | dissolved in culture media |
| Cell Storage: | incubator |
| Cell Growth Container: | petri dish (Corning) |
| Cell Growth Config: | monolayer, adherent |
| Cell Growth Rate: | 5-6 days |
| Cell Inoc Proc: | trypsinized, then subculture |
| Cell Media: | RPMI, EMEM |
| Cell Envir Cond: | 37 C, 4.5% CO2 incubator |
| Cell Harvesting: | after trypsinization |
| Cell Pct Confluence: | 80% |
| Cell Media Lastchanged: | 2-3 days |
