Summary of Study ST000173

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000150. The data can be accessed directly via it's Project DOI: 10.21228/M8KG68 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000173
Study TitleComparative metabolomics study of WT and iButOH tolerant E. coli
Study SummaryThis study aims to elucidate metabolic mechanisms of tolerance to isobutanol in E. coli. Two strains are utilized: WT parental strain EcHW24, and isobutanol tolerant strain 38-20-4 created via multiplex genome engineering. The metabolic response to growth with isobutanol (0.7% w/v) and without (0% w/v) on NG50 minimal media will be compared for each strain. 3 replicates for each strain/iButOH concentration have been submitted, excpet for WT/0.7%; we have observed high growth phenotype variation for this combination, and have corresponding submitted 5x replicates. Strain 38-20-4 contains mutations in the TCA cycle (gltA SNP) and amino acid metabolism (loss-of-function indels in glnE, gltD, and tnaA), thus our hypothesis is that tolerance arises via rewiring of TCA cycle and amino acid metabolism, possibly resulting in increased intracellular ratio of glutamine:glutamate
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-04
Num Groups4
Total Subjects14
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size2.0 G
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8KG68
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR000199
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