Summary of Study ST000247

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000199. The data can be accessed directly via it's Project DOI: 10.21228/M8P304 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000247
Study TitleDetermining the metabolic profile of wildtype and nos mutant Staphylococcus aureus grown in media lacking glucose using targeted LC/MS
Study TypeSingle time point
Study SummaryWhole cells from wildtype, nos mutant, SrrAB mutant, SrrAB nos double mutant, and complement strains will be isolated for targeted metabolite analysis. In parallel, supernatants (extracellular metabolites) will also be analyzed for their metabolic profile.
Institute
University of Florida
DepartmentSECIM
Last NameRice
First NameKelly
Emailkcrice@ufl.edu
Submit Date2015-02-17
Num Groups7
Total Subjects21
Raw Data File Type(s)raw(Thermo), d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Kelly Rice Kelly Rice
https://dx.doi.org/10.21228/M8P304
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR000278
Treatment Summary:nos mutation, SrrAB mutation, and nos SrrAB double mutant mutations were completed; as well as complementation before growing each strain under the same conditions. All strains were grown in Tryptic soy broth without dextrose. No additional treatment and/or variables are included in the experiment.
Treatment Protocol Filename:Rice_Treatments_targeted_20150807.txt
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