Summary of Study ST000289

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000231. The data can be accessed directly via it's Project DOI: 10.21228/M8SP4F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000289
Study TitlePPDK RNAi effects in endosperm metabolite pools
Study SummaryMaize plants were transformed with endosperm-specific PPDK RNAi knockout constructs to alter starch/protein ratios. Metabolite pool comparisons will be examined from sibling kernels harvested from segregating ears.
Institute
University of Florida
DepartmentSECIM
LaboratoryStewart
Last NameStewart
First NameJon
Address102 Leigh Hall
Emailjds2@chem.ufl.edu
Phone352-846-0743
Submit Date2015-08-17
Num Groups2
Total Subjects6
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Jon Stewart Jon Stewart
https://dx.doi.org/10.21228/M8SP4F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR000323
Treatment Summary:Each ear is segregating both mutant and wild type kernels, thus the harvest and freezing for the controls is the exact same as the mutant sibling kernels from the same ear.
Plant Growth Location:Woodruff Farm, Story Co., Ames, IA
Plant Plot Design:10 plants/row; random row placement over 0.5 acres
Plant Light Period:natural sunlight and day length, summer 2014
Plant Estab Date:2014-05-01
Plant Growth Stage:20 days after pollination
Plant Metab Quench Method:flash frozen in liquid N2, maintained at -80C
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