Summary of Study ST000561
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000412. The data can be accessed directly via it's Project DOI: 10.21228/M8SS33 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000561 |
| Study Title | Exploring the link between genotype, phenotype and metabolome for tomato seed quality attributes |
| Study Type | Tomato Seed Metabolites Profiling (dry seed and 6 hour imbibed seeds comparision) |
| Study Summary | In this study the F8 population of 100 Recombinant Inbred Lines (RILs) obtained from a cross between Solanum lycopersicum X Solanum pimpinellifolium were intelligently allocated to two sub-populations optimized for the distribution of parental alleles using the R-procedure DesignGG (Li et al., 2009; Joosen et al., 2013); hence 50 RIL lines were used for dry seeds and 50 lines for 6h imbibed seeds |
| Institute | Wageningen University & Research |
| Department | Plant Physiology |
| Laboratory | Wageningen Seed Lab, Lab |
| Last Name | Ligterink |
| First Name | Wilco |
| Address | Droevendaalsesteeg 1, NL-6708 PB Wageningen, The Netherlands |
| wilco.ligterink@wur.nl | |
| Phone | 31 317 48 28 09 |
| Submit Date | 2017-02-07 |
| Num Groups | 1 |
| Total Subjects | 100 |
| Publications | Metabolomic analysis of tomato seed germination, Metabolomics DOI: 10.1007/s11306-017-1284-x |
| Analysis Type Detail | GC-MS |
| Release Date | 2017-10-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000597 |
| Treatment Summary: | In this study the F8 population of 100 Recombinant Inbred Lines (RILs) obtained from a cross between Solanum lycopersicum X Solanum pimpinellifolium were intelligently allocated to two sub-populations optimized for the distribution of parental alleles using the R-procedure DesignGG (Li et al., 2009; Joosen et al., 2013); hence 50 RIL lines were used for dry seeds and 50 lines for 6h imbibed seeds |
| Treatment: | Metabolites were extracted from dry and 6 hour imbibed seeds |
| Treatment Route: | Seed |
| Plant Growth Location: | The S. lycopersicum × S. pimpinellifolium RIL population was grown twice under controlled conditions in the greenhouse facilities at Wageningen University, in the Netherlands. |
| Plant Plot Design: | RCBD |
| Plant Light Period: | 16h light and 8h dark (long-day conditions) |
| Plant Humidity: | 30% RH |
| Plant Temp: | The day and night temperatures were maintained at 25 °C and 15 °C, respectively |
| Plant Watering Regime: | Watered Daily |
| Plant Nutritional Regime: | All the RILs were uniformly supplied with the basic dose of fertilizer |
| Plant Growth Stage: | Seeds were extracted from healthy fruits and treated with 1% hydrochloric acid (HCL) to remove the large pieces of the pulp that were sticking onto the seeds. |
| Plant Storage: | The cleaned seeds were dried for 3d at 20 °C and were stored in a cool, dry storage room (13 °C and 30% RH) in paper bags |
