Summary of Study ST000890
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000619. The data can be accessed directly via it's Project DOI: 10.21228/M8NT1F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000890 |
Study Title | Identification of RXR Ligands |
Study Type | Idenfication of Ligands by HPLC-MS |
Study Summary | Free fatty acids in mouse plasma were identified and quantified by LC-MS. Through differential feeding and PHZ (phnylhydrazine) dosing, coupled with mass spectrometry, we identified the long chain fatty acid C24:5 as a natural RXRA ligand, which was dynamically increased in concentration in response to hematopoietic stress. Collectively, these data demonstrate that natural RXRA ligands are present and are dynamically regulated in vivo in mouse hematopoietic cells. |
Institute | Washington University in St. Louis |
Department | Diabetic Cardiovascular Disease Center, School of Medicine |
Laboratory | Metabolomics Core |
Last Name | Fujiwara |
First Name | Hideji |
Address | 660 South Euclid Ave, St. Louis MO 63110 |
hfujiwar@wustl.edu | |
Phone | 314-747-0494 |
Submit Date | 2017-09-22 |
Study Comments | Units of measurement:peak area ratio: analyte peak area/peak area of internal standard |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-22 |
Release Version | 1 |
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Treatment:
Treatment ID: | TR000941 |
Treatment Summary: | Phenylhydrazine was administered at 1mg once on day 1 and plasma was collected on day 4. Vitamin A deficient diet: Teklad, TD.86143. Control diet: Teklad, TD.91280. Fatty acid deficient diet: Teklad, TD.03314. Control diet: Teklad, TD.130321. |