Summary of Study ST000961

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000659. The data can be accessed directly via it's Project DOI: 10.21228/M8GX0J This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000961
Study Title	The Influence of Sugar, Artificial Sweeteners, and the Microbiome on Rodent Amino Acids (part IV)
Study Summary	Targeted Amino Acid panel of rodents treated with diets rich in commonly used artifically sweeteners will be assessed in this study. We hypothesized that a specific subset of plasma metabolites are generated as a result from a diet rich in commonly used artificial sweeteners and their subsequent processing by the gut microbiome, which could ultimately lead to impaired glycemic control and negative physiological health outcomes. To test this hypothesis we administered normal, high glucose, fructose, aspartame, and acesulfame potassium diets to rats for 3 weeks, followed by a plasma collection through cardiac puncture and metabolic analysis. We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals. The resulting data will give us insights into the influence of high sugar and artificial sweetener diets on homeostatic metabolic processes and dive into the symbiotic relationship of the gut microbiome with this process.
Institute	Mayo Clinic
Last Name	Hoffmann
First Name	Brian
Address	8701 Watertown Plank Road Milwaukee, WI 53226
Email	bhoffmann@mcw.edu
Phone	414-955-8671
Submit Date	2018-04-14
Analysis Type Detail	LC-MS
Release Date	2020-04-15
Release Version	1

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Treatment:

Treatment ID:	TR001014
Treatment Summary:	We administered normal, high glucose, aspartame, and acesulfame potassium diets to rats for 3 weeks, followed by a plasma collection through cardiac puncture and metabolic analysis (Group 1-4 samples). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group (Group 9). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group with antibiotic (Group 10). For the gut microbiota experiment, during the last 10 days of the diet subsets of all groups will have bacitracin and streptomycin (B/S) provided in their drinking water (0.5g/250 mL). The resulting data will give us insights into the influence of high sugar and artificial sweetener diets on homeostatic metabolic processes and dive into the symbiotic relationship of the gut microbiome with this process. Group1 = normal diet Group2 = high glucose diet Group3 = aspartame diet Group4 = acesulfame potassium diet Group5 = rat gut microbiota normal diet + antibotics Group6 = rat gut microbiota high glucose diet + antibotics Group7 = rat gut microbiota aspartame diet + antibotics Group8 = rat gut acesulfame potassium diet + antibotics Group9 = fructose diet Group10 = rat gut fructose diet + antibotics

Treatment ID:

TR001014

Treatment Summary:

We administered normal, high glucose, aspartame, and acesulfame potassium diets to rats for 3 weeks, followed by a plasma collection through cardiac puncture and metabolic analysis (Group 1-4 samples). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group (Group 9). We also treated the gut microbiota with in rats with the same diets plus bacitracin/streptomycin to observe how alterations of the microbiome influence the plasma metabolic profile in these animals (Groups 5-8). The samples here contain the fructose diet group with antibiotic (Group 10). For the gut microbiota experiment, during the last 10 days of the diet subsets of all groups will have bacitracin and streptomycin (B/S) provided in their drinking water (0.5g/250 mL). The resulting data will give us insights into the influence of high sugar and artificial sweetener diets on homeostatic metabolic processes and dive into the symbiotic relationship of the gut microbiome with this process. Group1 = normal diet Group2 = high glucose diet Group3 = aspartame diet Group4 = acesulfame potassium diet Group5 = rat gut microbiota normal diet + antibotics Group6 = rat gut microbiota high glucose diet + antibotics Group7 = rat gut microbiota aspartame diet + antibotics Group8 = rat gut acesulfame potassium diet + antibotics Group9 = fructose diet Group10 = rat gut fructose diet + antibotics