Summary of Study ST001134

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000759. The data can be accessed directly via it's Project DOI: 10.21228/M8K67W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001134
Study TitleGC-TOF-MS, Brassica nigra / MeJA + herbivory
Study SummaryGC-TOF-MS, Brassica nigra / MeJA + herbivory
Institute
Umeå Plant Science Centre
DepartmentPlant Physiology
Last NamePapazian
First NameStefano
AddressUmeå University
Emailstefano.papazian@umu.se
Phone0046723160854
Submit Date2019-02-06
Num Groups4
Total Subjects55
Study CommentsMeasurements are chromatographic peak area normalized by internal standard, arbritrary units.
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2019-08-06
Release Version1
Stefano Papazian Stefano Papazian
https://dx.doi.org/10.21228/M8K67W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR001210
Treatment Summary:Two types of treatment were used separately and in sequence: simulated herbivory with use of the phytohormone MeJA (M); real herbivory by P. brassicae caterpillars (P); and combined treatment (MP) where caterpillars were introduced to feed on (M)-plants for five days, after three days of MeJA pre-treatment. MeJA (Sigma-Aldrich, CAS Number 39924-52-2) was applied to the whole plant by spraying 5 mL (1mM, diluted in MilliQ water). First instar caterpillars (15 individuals per plant) were placed on the first fully expanded leaf (L4) of experimental plant and left to feed freely for five days.
Plant Light Period:16:8 hours light: dark cycle
Plant Humidity:50-70% RH
Plant Temp:20-22 °C
Plant Growth Stage:vegetative (4 weeks)
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